Storage method of peptides

Peptide health foods only need to be placed in a cool and dry place.
For peptide reagents and peptide drugs, they need to be stored in a dryer. Before exposing them to air, freeze-dried peptides can be left at room temperature. This will reduce the impact of humidity, and when freeze-drying is not possible, the best method is to store in small working sample sizes. For Cys containing compounds, Deoxygenation buffer is essential for the dissolution of Met or Trp peptide reagents, as these peptide reagents are easily oxidized by air. Before sealing the bottle, nitrogen or argon gas slowly flowing through the peptide reagents will also reduce the oxidation effect. Peptide reagents containing Gln or Asn are also prone to degradation, and all of these peptides have a limited lifespan compared to those without these problematic glycosides.

Peptides have a wide range of solubility. The main problem with peptide insolubility is the formation of secondary structures. Except for peptides with multiple hydrophobic residues, this occurs more significantly in peptides with multiple hydrophobic residues. Salt promotes the formation of secondary structures. We suggest dissolving peptides in sterile distilled water or deionized water first. If it is necessary to increase the dissolution rate, ultrasonic treatment can be used. There is still a problem with dissolution, adding a small amount of dilute acetic acid (10%) or ammonia water will facilitate dissolution.
To store peptide reagents for a long time, it is best to freeze dry them. Cold dried powder can be stored at -20 ℃ or lower for several years with little or no degradation. Peptides in solution are far from stable. Peptides are easily degraded by bacteria and should be dissolved in sterile purified water.
Peptide solutions containing Met, Cys, or Tyr residues have limited lifespan due to oxidation. It should be dissolved in anaerobic solvents. To prevent repeated freeze-thaw damage, it is recommended to dissolve excess peptides for the experiment, and store the remaining peptides in solid form.